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Impact of LRRK2-specific ArfGAP1 phosphorylation sites on Golgi complex integrity ArfGAP1 is known to promote the GTP hydrolysis activity of Arf1 that is required for dissociation of the COPI coatmer complex from Golgi-derived vesicles, a prerequisite for vesicle fusion with target compartments such as the ER [3739]

Samples were completely lysed using an ultrasonic homogenizer in an ice bath for 20 min and centrifuged at 14,000 rpm under 4C for 10 min

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